Horse HSP-70/HSPA9(HeatShock Protein 70) ELISA Kit

Biorbyt

Horse HSP-70/HSPA9(HeatShock Protein 70) ELISA Kit

10

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse HSP-70/HSPA9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse HSP-70/HSPA9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse HSP-70/HSPA9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Horse HSP-70/HSPA9 in the samples is then determined by comparing the OD of the samples to the standard curve

1.57-100 ng/mL

0.53 ng/mL

Research Use Only

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HeatShock Protein 70(HSP-70/HSPA9). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HeatShock Protein 70(HSP-70/HSPA9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HeatShock Protein 70(HSP-70/HSPA9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HeatShock Protein 70(HSP-70/HSPA9) in the samples is then determined by comparing the OD of the samples to the standard curve.

Equine

41116158