Horse NF-kappaBp105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit

Biorbyt

Horse NF-kappaBp105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit

10

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse NFKB-p105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse NFKB-p105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse NFKB-p105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Horse NFKB-p105 in the samples is then determined by comparing the OD of the samples to the standard curve

0.16-10 ng/mL

0.059 ng/mL

Research Use Only

Horse NF-kappaBp105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit

41116158