Hot Start Core Kit Apta+, Kit of aptamer-inhibited hot start DNA polymerase for high specificity, dNTPs and reaction buffer

Jena Bioscience

Hot Start Core Kit Apta+, Kit of aptamer-inhibited hot start DNA polymerase for high specificity, dNTPs and reaction buffer

5

Other Application

Research Use Only

5 unit/ul

Taq Core Kit Apta+ contains all reagents required for PCR (except template and primer) in one box combining simple handling with high flexibility. Taq Polymerase is recommended for routine PCR applications (up to 4 kb fragment length), high throughput PCR or genotyping. The buffer system guarantees robust and reliable amplification results in almost all PCR applications. The buffer contains a well-balanced ratio of potassium-, ammonium- and magnesium-ions to ensure high specificity and minimal by-product formation without the need of additional optimization steps. Ruby Buffer contains gel loading buffer and an inherent red dye allowing the direct loading of the PCR product into the gel. The red dye allows an easy visual control during PCR set-up and in combination with the density reagent the direct loading of the reaction product into the gel. The enzyme replicates DNA at 72 °C. It catalyzes the polymerization of nucleotides into duplex DNA in 5 to 3 direction in the presence of magnesium. It also possesses a 5 to 3 polymerization-dependent exonuclease replacement activity but lacks a 3 to 5 exonuclease (proof-reading) activity. Activation step Hot Start Polymerase Apta+ requires no prolonged heating or denaturing step. The polymerase inhibiting aptamer is quickly released at the increased temperature of thermal cycling.

-20°C

41116133