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Hot Start Core Kit Apta+, Kit of aptamer-inhibited hot start DNA polymerase for high specificity, dNTPs and reaction buffer

Research Use Only
PCR-215S
Jena Bioscience
Product group Assays
200 units
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Overview

  • Supplier
    Jena Bioscience
  • Product Name
    Hot Start Core Kit Apta+, Kit of aptamer-inhibited hot start DNA polymerase for high specificity, dNTPs and reaction buffer
  • Delivery Days Customer
    5
  • Applications
    Other Application
  • Certification
    Research Use Only
  • Concentration
    5 unit/ul
  • Scientific Description
    Taq Core Kit Apta+ contains all reagents required for PCR (except template and primer) in one box combining simple handling with high flexibility. Taq Polymerase is recommended for routine PCR applications (up to 4 kb fragment length), high throughput PCR or genotyping. The buffer system guarantees robust and reliable amplification results in almost all PCR applications. The buffer contains a well-balanced ratio of potassium-, ammonium- and magnesium-ions to ensure high specificity and minimal by-product formation without the need of additional optimization steps. Ruby Buffer contains gel loading buffer and an inherent red dye allowing the direct loading of the PCR product into the gel. The red dye allows an easy visual control during PCR set-up and in combination with the density reagent the direct loading of the reaction product into the gel. The enzyme replicates DNA at 72 °C. It catalyzes the polymerization of nucleotides into duplex DNA in 5 to 3 direction in the presence of magnesium. It also possesses a 5 to 3 polymerization-dependent exonuclease replacement activity but lacks a 3 to 5 exonuclease (proof-reading) activity. Activation step Hot Start Polymerase Apta+ requires no prolonged heating or denaturing step. The polymerase inhibiting aptamer is quickly released at the increased temperature of thermal cycling.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116133