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Western Blot analysis of 293T cells using HoxA5 Polyclonal Antibody
Western Blot analysis of 293T cells using HoxA5 Polyclonal Antibody
Western Blot analysis of 293T cells using HoxA5 Polyclonal Antibody

HOXA5 Antibody

CSB-PA009244
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHOXA5
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Overview

  • Supplier
    Cusabio
  • Product Name
    HOXA5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3202
  • Target name
    HOXA5
  • Target description
    homeobox A5
  • Target synonyms
    homeo box 1C; homeo box A5; homeobox protein Hox-1C; homeobox protein HOXA5; homeobox protein Hox-A5; HOX1; HOX1.3; HOX1C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20719
  • Protein Name
    Homeobox protein Hox-A5
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HOXA5 using anti-HOXA5 antibody (A04018-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXA5 antigen affinity purified polyclonal antibody (Catalog # A04018-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXA5 at approximately 29KD. The expected band size for HOXA5 is at 29KD.
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Immunohistochemical staining of human fallopian tube shows moderate nuclear positivity in glandular cells.
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