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WB analysis of K562 and RAW264.7 cell lysates using GTX87854 HOXD10 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of K562 and RAW264.7 cell lysates using GTX87854 HOXD10 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of K562 and RAW264.7 cell lysates using GTX87854 HOXD10 antibody. The lane on the right is blocked with the synthesized peptide.

HOXD10 antibody

GTX87854
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetHOXD10
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Overview

  • Supplier
    GeneTex
  • Product Name
    HOXD10 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3236
  • Target name
    HOXD10
  • Target description
    homeobox D10
  • Target synonyms
    homeo box 4D; homeo box D10; homeobox protein Hox-4D; homeobox protein Hox-4E; homeobox protein Hox-D10; HOX4; Hox-4.4; HOX4D; HOX4E
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28358
  • Protein Name
    Homeobox protein Hox-D10
  • Scientific Description
    This gene is a member of the Abd-B homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox D genes located on chromosome 2. The encoded nuclear protein functions as a sequence-specific transcription factor that is expressed in the developing limb buds and is involved in differentiation and limb development. Mutations in this gene have been associated with Wilms tumor and congenital vertical talus (also known as rocker-bottom foot deformity or congenital convex pes valgus) and/or a foot deformity resembling that seen in Charcot-Marie-Tooth disease. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HOXD10 using anti-HOXD10 antibody (A05257-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXD10 antigen affinity purified polyclonal antibody (Catalog # A05257-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXD10 at approximately 40 kDa. The expected band size for HOXD10 is at 38 kDa.
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