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Gel: 10+12%SDS-PAGE, Lysate: 30 ug, Lane: Mouse kidney tissue, Primary antibody: CSB-PA975571(HOXD11 Antibody) at dilution 1/1100, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 60 seconds
Gel: 10+12%SDS-PAGE, Lysate: 30 ug, Lane: Mouse kidney tissue, Primary antibody: CSB-PA975571(HOXD11 Antibody) at dilution 1/1100, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 60 seconds
Gel: 10+12%SDS-PAGE, Lysate: 30 ug, Lane: Mouse kidney tissue, Primary antibody: CSB-PA975571(HOXD11 Antibody) at dilution 1/1100, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 60 seconds

HOXD11 Antibody

CSB-PA975571
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetHOXD11
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Overview

  • Supplier
    Cusabio
  • Product Name
    HOXD11 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3237
  • Target name
    HOXD11
  • Target description
    homeobox D11
  • Target synonyms
    homeo box 4F; homeo box D11; homeobox protein Hox-4F; homeobox protein Hox-D11; HOX4; Hox-4.6, mouse, homolog of; HOX4F
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31277
  • Protein Name
    Homeobox protein Hox-D11
  • Scientific Description
    This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on chromosome 2. Deletions that remove the entire HOXD gene cluster or the 5 end of this cluster have been associated with severe limb and genital abnormalities. The product of the mouse Hoxd11 gene plays a role in forelimb morphogenesis.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HOXD11 using anti-HOXD11 antibody (A10676-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hek293 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXD11 antigen affinity purified polyclonal antibody (Catalog # A10676-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXD11 at approximately 45KD. The expected band size for HOXD11 is at 45KD.
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