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Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA15827A0Rb at dilution of 1:20
Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA15827A0Rb at dilution of 1:20
Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA15827A0Rb at dilution of 1:20

HPRT1 Antibody

CSB-PA15827A0RB
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHPRT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    HPRT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3251
  • Target name
    HPRT1
  • Target description
    hypoxanthine phosphoribosyltransferase 1
  • Target synonyms
    HGPRT; HGPRTase; HPRT; hypoxanthine-guanine phosphoribosyltransferase; hypoxanthine-guanine phosphoribosyltransferase 1; testicular tissue protein Li 89
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00492
  • Protein Name
    Hypoxanthine-guanine phosphoribosyltransferase
  • Scientific Description
    Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HPRT1 using anti-HPRT1 antibody (A00668-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HPRT1 antigen affinity purified polyclonal antibody (Catalog # A00668-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HPRT1 at approximately 25 kDa. The expected band size for HPRT1 is at 25-28 kDa.
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