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Western Blot analysis of K562 cells using HPA1 Polyclonal Antibody.
Western Blot analysis of K562 cells using HPA1 Polyclonal Antibody.
Western Blot analysis of K562 cells using HPA1 Polyclonal Antibody.

HPSE Antibody

CSB-PA005563
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetHPSE
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Overview

  • Supplier
    Cusabio
  • Product Name
    HPSE Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10855
  • Target name
    HPSE
  • Target description
    heparanase
  • Target synonyms
    endo-glucoronidase; heparanase; heparanase-1; HPA; HPA1; HPR1; HPSE1; HSE1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y251
  • Protein Name
    Heparanase
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Heparanase 1 using anti-Heparanase 1 antibody (PB9427). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Human Placenta Tissue Lysate at 50ug, Lane 3: A549 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Heparanase 1 antigen affinity purified polyclonal antibody (Catalog # PB9427) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Heparanase 1 at approximately 61 kDa. The expected band size for Heparanase 1 is at 61 kDa.
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