Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HRH1 antibody [HL3037] (GTX640475) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Boiled and unboiled MDA-MB-231 whole cell (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HRH1 antibody [HL3037] (GTX640475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640475/GTX640475_T-45432_20240705_WB_ub_24070822_136.webp "Boiled and unboiled MDA-MB-231 whole cell (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HRH1 antibody [HL3037] (GTX640475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![HRH1 antibody [HL3037] detects HRH1 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green: HRH1 stained by HRH1 antibody [HL3037] (GTX640475) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX640475/GTX640475_T-45432_20240628_ICC_IF_B_24070822_362.webp "HRH1 antibody [HL3037] detects HRH1 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green: HRH1 stained by HRH1 antibody [HL3037] (GTX640475) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![Non-transfected (–) and transfected (+) unboiled 5637 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HRH1 antibody [HL3037] (GTX640475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640475/GTX640475_45572_20250425_WB_shRNA_watermark_25042920_116.webp "Non-transfected (–) and transfected (+) unboiled 5637 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HRH1 antibody [HL3037] (GTX640475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Untreated (–) and treated (+) unboiled MDA-MB-231 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HRH1 antibody [HL3037] (GTX640475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640475/GTX640475_T-45432_20250425_WB_treatment_PNGaseF_25042920_553.webp "Untreated (–) and treated (+) unboiled MDA-MB-231 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HRH1 antibody [HL3037] (GTX640475) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.")
Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HRH1 antibody [HL3037] (GTX640475) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HRH1 antibody [HL3037]
GTX640475
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetHRH1
Overview
- SupplierGeneTex
- Product NameHRH1 antibody [HL3037]
- Delivery Days Customer7
- Application Supplier NoteWB: 1:500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3037
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3269
- Target nameHRH1
- Target descriptionhistamine receptor H1
- Target synonymsH1-R, H1R, HH1R, hisH1, histamine H1 receptor, histamine receptor, subclass H1
- HostRabbit
- IsotypeIgG
- Protein IDP35367
- Protein NameHistamine H1 receptor
- Scientific DescriptionHistamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. It has been associated with multiple processes, including memory and learning, circadian rhythm, and thermoregulation. It is also known to contribute to the pathophysiology of allergic diseases such as atopic dermatitis, asthma, anaphylaxis and allergic rhinitis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2015]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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