Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSC70) Monoclonal Antibody, Unconjugated (bsm-33211M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSC70) Monoclonal Antibody, Unconjugated (bsm-33211M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
HSC70 monoclonal Antibody
BSM-33211M
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHSPA8
Overview
- SupplierBioss Antibodies
- Product NameHSC70 monoclonal Antibody
- Delivery Days Customer16
- ApplicationsWestern Blot
- Applications SupplierWB(1:300-5000)
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID8C7
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID3312
- Target nameHSPA8
- Target descriptionheat shock protein family A (Hsp70) member 8
- Target synonymsconstitutive heat shock protein 70; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 72p; heat shock 70kd protein 10; heat shock 70kDa protein 8; heat shock cognate 71 kDa protein; heat shock cognate protein 54; HEL-33; HEL-S-72p; HSC54; HSC70; HSC71; HSP71; HSP73; HSPA10; LAP1; LAP-1; lipopolysaccharide-associated protein 1; LPS-associated protein 1; NIP71; N-myristoyltransferase inhibitor protein 71
- HostMouse
- IsotypeIgG
- Protein IDP11142
- Protein NameHeat shock cognate 71 kDa protein
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203