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ProductsBack/Hsc70/HSPA8 Monoclonal Antibody

Hsc70/HSPA8 Monoclonal Antibody

CAB0415
Assay Genie
TargetHSPA8
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Hsc70/HSPA8 Rabbit mAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3312
  • Target name
    HSPA8
  • Target description
    heat shock protein family A (Hsp70) member 8
  • Target synonyms
    HEL-33, HEL-S-72p, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP-1, LAP1, NIP71, heat shock cognate 71 kDa protein, LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70, epididymis luminal protein 33, epididymis secretory sperm binding protein Li 72p, heat shock 70kDa protein 8, heat shock 70kd protein 10, heat shock cognate protein 54, heat shock protein family A member 8, lipopolysaccharide-associated protein 1
  • Host
    Rabbit
  • Protein IDP11142
  • Protein Name
    Heat shock cognate 71 kDa protein
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Hsc70 Polyclonal Antibody (bs-5117R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
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Figure 1. Western blot analysis of Hsc70 using anti-Hsc70 antibody (PB9641). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: human U87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsc70 antigen affinity purified polyclonal antibody (Catalog # PB9641) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsc70 at approximately 71 kDa. The expected band size for Hsc70 is at 71 kDa.
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