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ProductsBack/Hsc70/HSPA8 Monoclonal Antibody

Hsc70/HSPA8 Monoclonal Antibody

CAB0415
Assay Genie
TargetHSPA8
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Hsc70/HSPA8 Rabbit mAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3312
  • Target name
    HSPA8
  • Target description
    heat shock protein family A (Hsp70) member 8
  • Target synonyms
    constitutive heat shock protein 70; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 72p; heat shock 70kd protein 10; heat shock 70kDa protein 8; heat shock cognate 71 kDa protein; heat shock cognate protein 54; HEL-33; HEL-S-72p; HSC54; HSC70; HSC71; HSP71; HSP73; HSPA10; LAP1; LAP-1; lipopolysaccharide-associated protein 1; LPS-associated protein 1; NIP71; N-myristoyltransferase inhibitor protein 71
  • Host
    Rabbit
  • Protein IDP11142
  • Protein Name
    Heat shock cognate 71 kDa protein
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Hsc70 Polyclonal Antibody (bs-5117R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
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Figure 1. Western blot analysis of Hsc70 using anti-Hsc70 antibody (PB9641). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: human U87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsc70 antigen affinity purified polyclonal antibody (Catalog # PB9641) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsc70 at approximately 71 kDa. The expected band size for Hsc70 is at 71 kDa.
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