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HSD17B3 antibody

GTX114480
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHSD17B3
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Overview

  • Supplier
    GeneTex
  • Product Name
    HSD17B3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.92 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3293
  • Target name
    HSD17B3
  • Target description
    hydroxysteroid 17-beta dehydrogenase 3
  • Target synonyms
    EDH17B3, SDR12C2, 17-beta-hydroxysteroid dehydrogenase type 3, 17-beta-HSD 3, 17-beta-HSD3, estradiol 17-beta-dehydrogenase 2, hydroxysteroid dehydrogenase 3, short chain dehydrogenase/reductase family 12C member 2, testicular 17-beta-hydroxysteroid dehydrogenase, testosterone 17-beta-dehydrogenase 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP37058
  • Protein Name
    17-beta-hydroxysteroid dehydrogenase type 3
  • Scientific Description
    This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Kobayashi H, Shirasawa N, Naito A. Estrogen synthesis in the stomach of Sprague-Dawley rats: comparison to Wistar rats. Exp Anim. 2021,70(1):63-72. doi: 10.1538/expanim.20-0089
    Read this paper
  • Feng X, Xia K, Ke Q, et al. Transplantation of encapsulated human Leydig-like cells: A novel option for the treatment of testosterone deficiency. Mol Cell Endocrinol. 2021,519:111039. doi: 10.1016/j.mce.2020.111039
    Read this paper
  • Zimmer BM, Howell ME, Wei Q, et al. Loss of exogenous androgen dependence by prostate tumor cells is associated with elevated glucuronidation potential. Horm Cancer. 2016,7(4):260-71. doi: 10.1007/s12672-016-0268-z
    Read this paper

Datasheet

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Figure 1. Western blot analysis of HSD17B3 using anti-HSD17B3 antibody (A04128-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B3 antigen affinity purified polyclonal antibody (Catalog # A04128-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B3 at approximately 40 kDa. The expected band size for HSD17B3 is at 40 kDa.
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