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HSD17B3 Antibody

LS-C663487
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetHSD17B3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HSD17B3 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3293
  • Target name
    HSD17B3
  • Target description
    hydroxysteroid 17-beta dehydrogenase 3
  • Target synonyms
    EDH17B3, SDR12C2, 17-beta-hydroxysteroid dehydrogenase type 3, 17-beta-HSD 3, 17-beta-HSD3, estradiol 17-beta-dehydrogenase 2, hydroxysteroid dehydrogenase 3, short chain dehydrogenase/reductase family 12C member 2, testicular 17-beta-hydroxysteroid dehydrogenase, testosterone 17-beta-dehydrogenase 3
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HSD17B3 using anti-HSD17B3 antibody (A04128-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B3 antigen affinity purified polyclonal antibody (Catalog # A04128-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B3 at approximately 40 kDa. The expected band size for HSD17B3 is at 40 kDa.
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