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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA240849(HSD17B8 Antibody) at dilution 1/55, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA240849(HSD17B8 Antibody) at dilution 1/55, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA240849(HSD17B8 Antibody) at dilution 1/55, on the right is treated with fusion protein. (Original magnification: x200)

HSD17B8 Antibody

CSB-PA240849
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHSD17B8
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Overview

  • Supplier
    Cusabio
  • Product Name
    HSD17B8 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7923
  • Target name
    HSD17B8
  • Target description
    hydroxysteroid 17-beta dehydrogenase 8
  • Target synonyms
    D6S2245E, FABG, FABGL, H2-KE6, HKE6, KE6, RING2, SDR30C1, dJ1033B10.9, (3R)-3-hydroxyacyl-CoA dehydrogenase, 17-beta-HSD 8, 17-beta-hydroxysteroid dehydrogenase 8, 3-ketoacyl-[acyl-carrier-protein] reductase alpha subunit, 3-oxoacyl-[acyl-carrier-protein] reductase, KAR alpha subunit, estradiol 17-beta-dehydrogenase 8, estrogen 17-oxidoreductase, ke-6, protein Ke6, really interesting new gene 2 protein, short chain dehydrogenase/reductase family 30C member 1, testosterone 17-beta-dehydrogenase 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92506
  • Protein Name
    (3R)-3-hydroxyacyl-CoA dehydrogenase
  • Scientific Description
    In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. The protein encoded by this gene is similar to Ke6 and is a member of the short-chain dehydrogenase superfamily. An alternatively spliced transcript of this gene has been detected, but the full-length nature of this variant has not been determined.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of HSD17B8 using anti-HSD17B8 antibody (A08741-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B8 antigen affinity purified polyclonal antibody (Catalog # A08741-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B8 at approximately 34 kDa. The expected band size for HSD17B8 is at 34 kDa.
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