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HSDL2 Antibody

LS-C830993
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetHSDL2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HSDL2 Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:10000), IHC (1:50 - 1:100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID84263
  • Target name
    HSDL2
  • Target description
    hydroxysteroid dehydrogenase like 2
  • Target synonyms
    C9orf99, SDR13C1, hydroxysteroid dehydrogenase-like protein 2, short chain dehydrogenase/reductase family 13C member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western blot All lanes: HSDL2 antibody at 3microg/ml Lane 1: 293T whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 46, 38 kDa Observed band size: 46 kDa
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ICC/IF analysis of HDFn cells using GTX30325 HSDL2 antibody [2A11BG6AH6]. Fixation : 4% paraformaldehyde 20 mins Permeabilization : Triton X-100 (0.1%, 15 min) Dilution : 10 microg/ml overnight at 4oC
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Figure 1. Western blot analysis of HSDL2 using anti-HSDL2 antibody (A11317-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSDL2 antigen affinity purified polyclonal antibody (Catalog # A11317-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSDL2 at approximately 45 kDa. The expected band size for HSDL2 is at 45 kDa.
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Immunohistochemical staining of human colon, kidney, liver and lymph node using Anti-HSDL2 antibody HPA050453 (A) shows similar protein distribution across tissues to independent antibody HPA042890 (B).
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