More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/HSF1 Antibody
Western Blot analysis of COLO 293T cells using HSF1 Polyclonal Antibody
Western Blot analysis of COLO 293T cells using HSF1 Polyclonal Antibody
Western Blot analysis of COLO 293T cells using HSF1 Polyclonal Antibody

HSF1 Antibody

CSB-PA002974
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetHSF1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Cusabio
  • Product Name
    HSF1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3297
  • Target name
    HSF1
  • Target description
    heat shock transcription factor 1
  • Target synonyms
    HSTF1, heat shock factor protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00613
  • Protein Name
    Heat shock factor protein 1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
MedChem Express
Phospho-HSF1 (Ser303/Ser307) Antibody (YA188, Rabbit)HY-P80822
TargetHSF1
  • SizePrice
Anti-HSF1 Antibody
Product group Antibodies
Antibodies.com
Anti-HSF1 AntibodyA98093
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Figure 1. Western blot analysis of HSF1 using anti-HSF1 antibody (A00250). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF1 antigen affinity purified polyclonal antibody (Catalog # A00250) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSF1 at approximately 80 kDa. The expected band size for HSF1 is at 57 kDa.
Product group Antibodies
Boster Bio
Anti-HSF1 Antibody Picoband(r)A00250-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetHSF1
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-HSF1 Antibody, Affinity PurifiedA303-174A
ApplicationsImmunoPrecipitation, ImmunoHistoChemistry
TargetHSF1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-HSF1 Antibody144-60474
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetHSF1
  • SizePrice
Product group Antibodies
Abbexa
HSF1 Sumoylation Site AntibodyABX031603
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • SizePrice
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-HSF1 Polyclonal Antibody, Unconjugated (bs-3757R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Product group Antibodies
Bioss
HSF1 Polyclonal AntibodyBS-3757R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetHSF1
  • SizePrice
Product group Antibodies
Assay Genie
HSF1 AntibodyCAB13765
TargetHSF1
  • SizePrice
Product group Antibodies
Biomatik
HSF1 Polyclonal AntibodyCAC14986
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetHSF1
  • SizePrice
ICC/IF analysis of L929 cells using GTX04342 HSF1 antibody. Green : Primary antibody Blue : DAPI Dilution : 1:100
Product group Antibodies
GeneTex
HSF1 antibodyGTX04342
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetHSF1
  • SizePrice