More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/HSP27 Polyclonal Antibody
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-HSP27/HspB1/HSP25 Polyclonal Antibody, Unconjugated (bs-0730R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-HSP27/HspB1/HSP25 Polyclonal Antibody, Unconjugated (bs-0730R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-HSP27/HspB1/HSP25 Polyclonal Antibody, Unconjugated (bs-0730R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

HSP27 Polyclonal Antibody

BS-0730R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHSPB1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    HSP27 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3315
  • Target name
    HSPB1
  • Target description
    heat shock protein family B (small) member 1
  • Target synonyms
    28 kDa heat shock protein; CMT2F; epididymis secretory protein Li 102; estrogen-regulated 24 kDa protein; heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; heat shock protein beta-1; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; putative heat shock protein family B member 1 isoform; SRP27; stress-responsive protein 27
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04792
  • Protein Name
    Heat shock protein beta-1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Indolyl-chalcone derivatives induce hepatocellular carcinoma cells apoptosis through oxidative stress related mitochondrial pathway in vitro and in vivo. Zhang X et al., 2018 Sep 25, Chem Biol Interact
    Read more
  • Expression of Heat Shock Protein-27 (Hsp27) and P38MAPK in Esophageal Squamous Cell Carcinoma. Zhang Y et al., 2017 Nov 3, Med Sci Monit
    Read more

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Bethyl Laboratories
Rabbit anti-HSP27 Antibody, Affinity PurifiedA304-709A
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
TargetHSPB1
  • SizePrice
Anti-HSP27 Antibody
Product group Antibodies
Antibodies.com
Anti-HSP27 AntibodyA100672
ApplicationsELISA, ImmunoHistoChemistry
  • SizePrice
Figure 1. Western blot analysis of HSP27 using anti-HSP27 antibody (PB9237). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human HT1080 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP27 antigen affinity purified polyclonal antibody (Catalog # PB9237) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP27 at approximately 27 kDa. The expected band size for HSP27 is at 27 kDa.
Product group Antibodies
Boster Bio
Anti-Hsp27/HSPB1 Antibody Picoband(r)PB9237-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
TargetHSPB1
  • SizePrice
Product group Antibodies
Biomatik
HSPB1 Polyclonal AntibodyCAC15125
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetHSPB1
  • SizePrice
Product group Antibodies
Assay Genie
HSP27/HSPB1 AntibodyCAB0240
TargetHSPB1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-HSP27 Antibody130-10055
ApplicationsELISA
TargetHSPB1
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-HSPB1 [SAIC-23B-22]AB00313-1.1-BT
ApplicationsMass Spectrometry
TargetHSPB1
  • SizePrice
Product group Antibodies
Cusabio
HSPB1 Monoclonal AntibodyCSB-MA080263
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetHSPB1
  • SizePrice
Immunohistochemistry analysis in human skin and pancreas tissues using Anti-HSPB1 antibody. Corresponding HSPB1 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-HSPB1 AntibodyHPA000497
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetHSPB1
  • SizePrice
Product group Antibodies
References
GeneTex
HSP27 antibodyGTX101145
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetHSPB1
  • SizePrice