IHC-P analysis of human breast carcinoma tissue using GTX25478 HSP60 antibody [4B9/89]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50
![IHC-P analysis of human kidney tissue using GTX25478 HSP60 antibody [4B9/89]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_1293_IHC-P_w_23060722_862.webp "IHC-P analysis of human kidney tissue using GTX25478 HSP60 antibody [4B9/89]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100")
![IHC-P analysis of human tonsil tissue using GTX25478 HSP60 antibody [4B9/89]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_1294_IHC-P_w_23060722_594.webp "IHC-P analysis of human tonsil tissue using GTX25478 HSP60 antibody [4B9/89]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100")
![IP analysis of HeLa cell lysates using GTX25478 HSP60 antibody [4B9/89]. IP reaction : 2microg antibody / 500microg lysate Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_1445_IP_w_23060722_852.webp "IP analysis of HeLa cell lysates using GTX25478 HSP60 antibody [4B9/89]. IP reaction : 2microg antibody / 500microg lysate Dilution : 1:1000")
![FACS analysis of permeabilized, fixed HeLa cells using GTX25478 HSP60 antibody [4B9/89]. Orange histogram : Primary antibody Black histogram : Isotype control antibody Dilution : 10 microg/ml incubated for 1 hour on ice](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_176_FACS_w_23060722_874.webp "FACS analysis of permeabilized, fixed HeLa cells using GTX25478 HSP60 antibody [4B9/89]. Orange histogram : Primary antibody Black histogram : Isotype control antibody Dilution : 10 microg/ml incubated for 1 hour on ice")
![WB analysis of 50microg of the indicated whole cell lysates using GTX25478 HSP60 antibody [4B9/89]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_1782_WB_w_23060722_184.webp "WB analysis of 50microg of the indicated whole cell lysates using GTX25478 HSP60 antibody [4B9/89]. Dilution : 1:1000")
![ICC/IF analysis of HeLa and NIH3T3 cells using GTX25478 HSP60 antibody [4B9/89]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_660_ICC-IF_w_23060722_651.webp "ICC/IF analysis of HeLa and NIH3T3 cells using GTX25478 HSP60 antibody [4B9/89]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature")
![ICC/IF analysis of HeLa cells using GTX25478 HSP60 antibody [4B9/89]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_661_ICC-IF_w_23060722_354.webp "ICC/IF analysis of HeLa cells using GTX25478 HSP60 antibody [4B9/89]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature")
![ICC/IF analysis of human endothelial cells using GTX25478 HSP60 antibody [4B9/89].](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_662_ICC-IF_w_23060722_232.webp "ICC/IF analysis of human endothelial cells using GTX25478 HSP60 antibody [4B9/89].")
![ICC/IF analysis of A2058 Cells using GTX25478 HSP60 antibody [4B9/89]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_663_ICC-IF_w_23060722_781.webp "ICC/IF analysis of A2058 Cells using GTX25478 HSP60 antibody [4B9/89]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
IHC-P analysis of human breast carcinoma tissue using GTX25478 HSP60 antibody [4B9/89]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50
HSP60 antibody [4B9/89]
GTX25478
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
ReactivityBacteria, Human, Mouse, Primate, Rat
TargetHSPD1
Overview
- SupplierGeneTex
- Product NameHSP60 antibody [4B9/89]
- Delivery Days Customer9
- Antibody SpecificityEpitope mapping studies using human HSP60 deletion mutants suggest that this antibody binds either between amino acids 335-366 or 484-547.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4B9/89
- ConjugateUnconjugated
- Gene ID3329
- Target nameHSPD1
- Target descriptionheat shock protein family D (Hsp60) member 1
- Target synonyms60 kDa chaperonin; 60 kDa heat shock protein, mitochondrial; chaperonin 60; CPN60; epididymis secretory sperm binding protein; GROEL; heat shock 60kDa protein 1 (chaperonin); heat shock protein 65; HLD4; HSP60; HSP-60; HSP65; HuCHA60; mitochondrial matrix protein P1; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; SPG13
- HostMouse
- IsotypeIgG2a
- Scientific DescriptionThis gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
- ReactivityBacteria, Human, Mouse, Primate, Rat
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203
References
- Overexpression of the mitochondrial pyruvate carrier reduces lactate production and increases recombinant protein productivity in CHO cells. Bulte DB et al., 2020 Sep, Biotechnol BioengRead more
- Synergistic and differential modulation of immune responses by Hsp60 and lipopolysaccharide. Osterloh A et al., 2007 Feb 16, J Biol ChemRead more