![IHC-P analysis of human breast carcinoma tissue using GTX25478 HSP60 antibody [4B9/89]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50 IHC-P analysis of human breast carcinoma tissue using GTX25478 HSP60 antibody [4B9/89]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_1292_IHC-P_w_23060722_302.webp)
IHC-P analysis of human breast carcinoma tissue using GTX25478 HSP60 antibody [4B9/89]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50
HSP60 antibody [4B9/89]
GTX25478
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
TargetHSPD1
Overview
- SupplierGeneTex
- Product NameHSP60 antibody [4B9/89]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:100 - 1:1000. ICC/IF: 10 microg/ml. IHC-P: 1:200. FACS: 1-20 microg/ml. IP: 2microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4B9/89
- ConjugateUnconjugated
- Gene ID3329
- Target nameHSPD1
- Target descriptionheat shock protein family D (Hsp60) member 1
- Target synonymsCPN60, GROEL, HLD4, HSP-60, HSP60, HSP65, HuCHA60, SPG13, 60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, P60 lymphocyte protein, chaperonin 60, epididymis secretory sperm binding protein, heat shock 60kDa protein 1 (chaperonin), heat shock protein 65, heat shock protein family D member 1, mitochondrial matrix protein P1, short heat shock protein 60 Hsp60s1
- HostMouse
- IsotypeIgG2a
- Protein IDP10809
- Protein Name60 kDa heat shock protein, mitochondrial
- Scientific DescriptionThis gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Overexpression of the mitochondrial pyruvate carrier reduces lactate production and increases recombinant protein productivity in CHO cells. Bulte DB et al., 2020 Sep, Biotechnol BioengRead more
- Synergistic and differential modulation of immune responses by Hsp60 and lipopolysaccharide. Osterloh A et al., 2007 Feb 16, J Biol ChemRead more
![IHC-P analysis of human kidney tissue using GTX25478 HSP60 antibody [4B9/89]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100 IHC-P analysis of human kidney tissue using GTX25478 HSP60 antibody [4B9/89]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_1293_IHC-P_w_23060722_862.webp)
![IHC-P analysis of human tonsil tissue using GTX25478 HSP60 antibody [4B9/89]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100 IHC-P analysis of human tonsil tissue using GTX25478 HSP60 antibody [4B9/89]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_1294_IHC-P_w_23060722_594.webp)
![IP analysis of HeLa cell lysates using GTX25478 HSP60 antibody [4B9/89]. IP reaction : 2microg antibody / 500microg lysate Dilution : 1:1000 IP analysis of HeLa cell lysates using GTX25478 HSP60 antibody [4B9/89]. IP reaction : 2microg antibody / 500microg lysate Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_1445_IP_w_23060722_852.webp)
![FACS analysis of permeabilized, fixed HeLa cells using GTX25478 HSP60 antibody [4B9/89]. Orange histogram : Primary antibody Black histogram : Isotype control antibody Dilution : 10 microg/ml incubated for 1 hour on ice FACS analysis of permeabilized, fixed HeLa cells using GTX25478 HSP60 antibody [4B9/89]. Orange histogram : Primary antibody Black histogram : Isotype control antibody Dilution : 10 microg/ml incubated for 1 hour on ice](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_176_FACS_w_23060722_874.webp)
![WB analysis of 50microg of the indicated whole cell lysates using GTX25478 HSP60 antibody [4B9/89]. Dilution : 1:1000 WB analysis of 50microg of the indicated whole cell lysates using GTX25478 HSP60 antibody [4B9/89]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_1782_WB_w_23060722_184.webp)
![ICC/IF analysis of HeLa and NIH3T3 cells using GTX25478 HSP60 antibody [4B9/89]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature ICC/IF analysis of HeLa and NIH3T3 cells using GTX25478 HSP60 antibody [4B9/89]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_660_ICC-IF_w_23060722_651.webp)
![ICC/IF analysis of HeLa cells using GTX25478 HSP60 antibody [4B9/89]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature ICC/IF analysis of HeLa cells using GTX25478 HSP60 antibody [4B9/89]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_661_ICC-IF_w_23060722_354.webp)
![ICC/IF analysis of human endothelial cells using GTX25478 HSP60 antibody [4B9/89]. ICC/IF analysis of human endothelial cells using GTX25478 HSP60 antibody [4B9/89].](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_662_ICC-IF_w_23060722_232.webp)
![ICC/IF analysis of A2058 Cells using GTX25478 HSP60 antibody [4B9/89]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC ICC/IF analysis of A2058 Cells using GTX25478 HSP60 antibody [4B9/89]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_663_ICC-IF_w_23060722_781.webp)

![IHC-P analysis of human breast carcinoma tissue using GTX25479 HSP60 antibody [2E1/53]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX25479/GTX25479_1295_IHC-P_w_23060722_125.webp)

![ELISA analysis of antigen using GTX60424 HSP60 antibody [3G8]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60424/GTX60424_20170912_ELISA_w_23061123_845.webp)

![FACS analysis of paraformaldehyde-fixed HeLa cells using GTX34785 HSP60 antibody [LK1]. Blue : Primary antibody Red : Isotype control](https://www.genetex.com/upload/website/prouct_img/normal/GTX34785/GTX34785_20200115_FACS_1653_w_23060801_985.webp)
![ICC/IF analysis of PFA-fixed MCF-7 cells using GTX34786 HSP60 antibody [SPM253]. Green : Primary antibody Red : nucleus](https://www.genetex.com/upload/website/prouct_img/normal/GTX34786/GTX34786_20200115_ICC-IF_1743_w_23060801_701.webp)
![ICC/IF analysis of HeLa cells using GTX34787 HSP60 antibody [GROEL/730]. Green : Primary antibody Red : nucleus](https://www.genetex.com/upload/website/prouct_img/normal/GTX34787/GTX34787_20200115_ICC-IF_222_w_23060801_266.webp)
![ICC/IF analysis of MeOH-fixed HeLa cells using GTX34788 HSP60 antibody [HSPD1/780]. Green : Primary antibody Red : nucleus](https://www.genetex.com/upload/website/prouct_img/normal/GTX34788/GTX34788_20200115_ICC-IF_1586_w_23060801_947.webp)