![Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Hsp90 beta antibody [N2C2], Internal (GTX101423) at 1:300 dilution. Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Hsp90 beta antibody [N2C2], Internal (GTX101423) at 1:300 dilution.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101423/GTX101423_39728_IHC_22111422_584.webp)
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Hsp90 beta antibody [N2C2], Internal (GTX101423) at 1:300 dilution.
Hsp90 beta antibody [N2C2], Internal
GTX101423
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityDrosophila, Human, Mouse, Rat, Zebra Fish
TargetHSP90AB1
Overview
- SupplierGeneTex
- Product NameHsp90 beta antibody [N2C2], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration5.43 mg/ml
- ConjugateUnconjugated
- Gene ID3326
- Target nameHSP90AB1
- Target descriptionheat shock protein 90 alpha family class B member 1
- Target synonymsD6S182, HSP84, HSP90B, HSPC2, HSPCB, heat shock protein HSP 90-beta, HSP90-beta, heat shock 84 kDa, heat shock 90kD protein 1, beta, heat shock protein 90 kDa, heat shock protein 90kDa alpha (cytosolic), class B member 1, heat shock protein 90kDa alpha family class B member 1, heat shock protein family C member 3
- HostRabbit
- IsotypeIgG
- Protein IDP08238
- Protein NameHeat shock protein HSP 90-beta
- Scientific DescriptionHSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1 (MIM 140571), an inducible form, and HSP90AB1, a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]
- ReactivityDrosophila, Human, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![Hsp90 beta antibody [N2C2], Internal detects Hsp90 beta protein at cytosol on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. Hsp90 beta antibody [N2C2], Internal (GTX101423) dilution: 1:200. Hsp90 beta antibody [N2C2], Internal detects Hsp90 beta protein at cytosol on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. Hsp90 beta antibody [N2C2], Internal (GTX101423) dilution: 1:200.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101423/GTX101423_39728_IHC-Wm_Z_22111422_490.webp)

![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 beta antibody [N2C2], Internal (GTX101423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 beta antibody [N2C2], Internal (GTX101423) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101423/GTX101423_39728_20230203_WB_Drosophila_23020621_556.webp)
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody [N2C2], Internal (GTX101423) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody [N2C2], Internal (GTX101423) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101423/GTX101423_39728_20170706_WB_R_w_23060100_972.webp)
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody [N2C2], Internal (GTX101423) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP90 beta antibody [N2C2], Internal (GTX101423) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101423/GTX101423_39728_20170706_WB_M_w_23060100_185.webp)
![HSP90 beta antibody [N2C2], Internal detects HSP90 beta protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. HSP90 beta antibody [N2C2], Internal (GTX101423) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min HSP90 beta antibody [N2C2], Internal detects HSP90 beta protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. HSP90 beta antibody [N2C2], Internal (GTX101423) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX101423/GTX101423_39728_IHC_R_w_23060100_354.webp)

![HSP90 beta antibody [N2C2], Internal detects HSP90AB1 protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. HSP90 beta antibody [N2C2], Internal (GTX101423) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min HSP90 beta antibody [N2C2], Internal detects HSP90AB1 protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. HSP90 beta antibody [N2C2], Internal (GTX101423) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX101423/GTX101423_39728_IHC_M_w_23060100_884.webp)





