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Western Blot analysis of HeLa cells using HSP105 Polyclonal Antibody
Western Blot analysis of HeLa cells using HSP105 Polyclonal Antibody
Western Blot analysis of HeLa cells using HSP105 Polyclonal Antibody

HSPH1 Antibody

CSB-PA002984
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetHSPH1
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Overview

  • Supplier
    Cusabio
  • Product Name
    HSPH1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10808
  • Target name
    HSPH1
  • Target description
    heat shock protein family H (Hsp110) member 1
  • Target synonyms
    antigen NY-CO-25; heat shock 105kDa/110kDa protein 1; heat shock protein 105 kDa; HSP105; HSP105A; HSP105B; NY-CO-25
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92598
  • Protein Name
    Heat shock protein 105 kDa
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Cell lysates of HeLa(30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hsp105 alpha (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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Figure 3. IHC analysis of Hsp105 using anti-Hsp105 antibody (A04168). Hsp105 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2microg/ml rabbit anti-Hsp105 Antibody (A04168) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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