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Immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using HTR2B Polyclonal Antibody at dilution 1:60
Immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using HTR2B Polyclonal Antibody at dilution 1:60
Immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using HTR2B Polyclonal Antibody at dilution 1:60

HTR2B Polyclonal Antibody

E-AB-10115
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    HTR2B Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.2mg/ml
  • Scientific Description
    This gene encodes one of the several different receptors for 5-hydroxytryptamine (serotonin) that belongs to the G-protein coupled receptor 1 family. Serotonin is a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. Serotonin receptors mediate many of the central and peripheral physiologic functions of serotonin, including regulation of cardiovascular functions and impulsive behavior. Population and family-based analyses of a minor allele (glutamine-to-stop substitution, designated Q20*) which blocks expression of this protein, and knockout studies in mice, suggest a role for this gene in impulsivity. However, other factors, such as elevated testosterone levels, may also be involved.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of 5HT2B Receptor using anti-5HT2B Receptor antibody (PB9600). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: U87 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-5HT2B Receptor antigen affinity purified polyclonal antibody (Catalog # PB9600) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 5HT2B Receptor at approximately 54 kDa. The expected band size for 5HT2B Receptor is at 54 kDa.
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