More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/HTRA1 Antibody
IHC image of CSB-PA835695LA01HU diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA835695LA01HU diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA835695LA01HU diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

HTRA1 Antibody

CSB-PA835695LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHTRA1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Cusabio
  • Product Name
    HTRA1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5654
  • Target name
    HTRA1
  • Target description
    HtrA serine peptidase 1
  • Target synonyms
    ARMD7, CADASIL2, CARASIL, CARASIL2, HtrA, L56, ORF480, PRSS11, serine protease HTRA1, IGFBP5-protease, high-temperature requirement A serine peptidase 1, protease, serine, 11 (IGF binding)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92743
  • Protein Name
    Serine protease HTRA1
  • Scientific Description
    Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Biomatik
High Temperature Requirement Factor A1 (HTRA1) Polyclonal AntibodyCAU21446
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetHTRA1
  • SizePrice
Product group Antibodies
Bioss
HTRA1 Recombinant AntibodyBSM-62786R
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetHTRA1
  • SizePrice
Product group Antibodies
Assay Genie
HTRA1 AntibodyCAB11693
TargetHTRA1
  • SizePrice
Anti-HTRA1 Antibody
Product group Antibodies
Antibodies.com
Anti-HTRA1 AntibodyA42545
ApplicationsWestern Blot
  • SizePrice
Figure 1. Western blot analysis of HTRA1 using anti-HTRA1 antibody (A01801-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTRA1 antigen affinity purified polyclonal antibody (Catalog # A01801-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HTRA1 at approximately 51KD. The expected band size for HTRA1 is at 51KD.
Product group Antibodies
Boster Bio
Anti-HTRA1 Antibody Picoband(r)A01801-1-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetHTRA1
  • SizePrice
Product group Antibodies
GeneTex
HtrA1 antibody [HL3709]GTX641881
ApplicationsWestern Blot
TargetHTRA1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
HTRA1 AntibodyLS-C832624
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetHTRA1
  • SizePrice
Immunofluorescent staining of human cell line LHCN-M2 shows localization to plasma membrane.
Product group Antibodies
Atlas Antibodies
Anti-HTRA1 AntibodyHPA036655
ApplicationsImmunoCytoChemistry
ReactivityHuman
TargetHTRA1
  • SizePrice