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ProductsBack/HTRA1 Rabbit Polyclonal Antibody
WB Suggested Anti-HTRA1 Antibody Titration: 1.0 ug/ml Positive Control: Fetal Kidney
WB Suggested Anti-HTRA1 Antibody Titration: 1.0 ug/ml Positive Control: Fetal Kidney
WB Suggested Anti-HTRA1 Antibody Titration: 1.0 ug/ml Positive Control: Fetal Kidney

HTRA1 Rabbit Polyclonal Antibody

TA358468
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadMSDS
Product group Antibodies
TargetHTRA1
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    HTRA1 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5654
  • Target name
    HTRA1
  • Target description
    HtrA serine peptidase 1
  • Target synonyms
    ARMD7; CADASIL2; CARASIL; high-temperature requirement A serine peptidase 1; HtrA; IGFBP5-protease; L56; ORF480; protease, serine, 11 (IGF binding); PRSS11; serine protease HTRA1
  • Host
    Rabbit
  • Protein IDQ92743
  • Protein Name
    Serine protease HTRA1
  • Scientific Description
    HTRA1 Antibody - N-terminal region
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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IHC image of CSB-PA835695LA01HU diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of HTRA1 using anti-HTRA1 antibody (A01801-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTRA1 antigen affinity purified polyclonal antibody (Catalog # A01801-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HTRA1 at approximately 51KD. The expected band size for HTRA1 is at 51KD.
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