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WB analysis of 293T, MCF7, HT-29, HL-60, HeLa, mouse brain, and rat brain lysates using HtrA2/Omi antibody at a dilution of 1:1,000.
WB analysis of 293T, MCF7, HT-29, HL-60, HeLa, mouse brain, and rat brain lysates using HtrA2/Omi antibody at a dilution of 1:1,000.
WB analysis of 293T, MCF7, HT-29, HL-60, HeLa, mouse brain, and rat brain lysates using HtrA2/Omi antibody at a dilution of 1:1,000.

HTRA2 antibody [1B3]

GTX53729
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetHTRA2
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Overview

  • Supplier
    GeneTex
  • Product Name
    HTRA2 antibody [1B3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1B3
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID27429
  • Target name
    HTRA2
  • Target description
    HtrA serine peptidase 2
  • Target synonyms
    epididymis secretory sperm binding protein; high temperature requirement protein A2; HtrA-like serine protease; MGCA8; OMI; Omi stress-regulated endoprotease; PARK13; protease, serine, 25; PRSS25; serine protease 25; serine protease HTRA2, mitochondrial; serine proteinase OMI
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO43464
  • Protein Name
    Serine protease HTRA2, mitochondrial
  • Scientific Description
    This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HTRA2 using anti-HTRA2 antibody (A01941-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HT-1080 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTRA2 antigen affinity purified polyclonal antibody (Catalog # A01941-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HTRA2 at approximately 36 kDa. The expected band size for HTRA2 is at 36,49 kDa.
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