Human 5-HT (5-Hydroxytryptamine) ELISA Kit

Assay Genie

Human 5-HT (5-Hydroxytryptamine) ELISA Kit

12

ELISA

14.07-900 ng/mL

4.05 ng/mL

Research Use Only

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 5-HT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.

2°C to 8°C

41116100