Functional ELISA analysis of immobilized recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
![Indirect ELISA analysis performed by coating plate with recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag protein (GTX135683-pro) (15.63-1000 ng/mL). Coated protein probed with ACE2 antibody [N1N2], N-term (GTX101395) (0.1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX135683-pro/GTX135683-pro_43973_20200626_ELISA_Indirect_w_23060620_286.webp "Indirect ELISA analysis performed by coating plate with recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag protein (GTX135683-pro) (15.63-1000 ng/mL). Coated protein probed with ACE2 antibody [N1N2], N-term (GTX101395) (0.1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.")
![Inhibition analysis of immobilized recombinant RBD proteins derived from different strains of SARS-CoV-2 virus (Wild type or B1.617.2 delta variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL) ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).](https://www.genetex.com/upload/website/prouct_img/normal/GTX135683-pro/GTX135683-pro_44309_20210728_Neutralizing_Inhibition_Multiple_w_23060620_310.webp "Inhibition analysis of immobilized recombinant RBD proteins derived from different strains of SARS-CoV-2 virus (Wild type or B1.617.2 delta variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL) ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).")
![GTX135683-pro Human ACE2 (ECD) protein , mouse IgG Fc tag protein (1 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX135683-pro/GTX135683-pro_43973_20200619_WB_Ag_w_23060620_535.webp "GTX135683-pro Human ACE2 (ECD) protein , mouse IgG Fc tag protein (1 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).](https://www.genetex.com/upload/website/prouct_img/normal/GTX135683-pro/GTX135683-pro_44309_20210712_Neutralizing_Inhibition_w_23060620_267.webp "Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).")
Functional ELISA analysis of immobilized recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
Human ACE2 (ECD) protein, mouse IgG Fc tag (active)
GTX135683-PRO
Product group Molecular Biology
Overview
- SupplierGeneTex
- Product NameHuman ACE2 (ECD) protein, mouse IgG Fc tag (active)
- Delivery Days Customer9
- ApplicationsFunctional Assay, ELISA, Other Application
- CertificationResearch Use Only
- Concentration0.8 mg/ml
- ConjugateUnconjugated
- Scientific DescriptionThe protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]
- Storage Instruction-20°C,-80°C
- UNSPSC12352204