Bio-Connect

Human Anti-MPV IgG (Anti-Monkeypox Virus IgG) ELISA Kit

ORB2307997
Biorbyt
ReactivityHuman
Product group Assays
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Human Anti-MPV IgG (Anti-Monkeypox Virus IgG) ELISA Kit
  • Delivery Days Customer
    10
  • Application Supplier Note
    standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-MPV IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-MPV IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-MPV IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human Anti-MPV IgG in the samples is then determined by comparing the OD of the samples to the standard curve
  • Assay Detection Range
    3.13-200 ng/mL
  • Assay Sensitivity
    1.39 ng/mL
  • Certification
    Research Use Only
  • Scientific Description
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-MPV IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-MPV IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-MPV IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human Anti-MPV IgG in the samples is then determined by comparing the OD of the samples to the standard curve.
  • Reactivity
    Human
  • UNSPSC
    41116158