Human Anti-MPV IgG (Anti-Monkeypox Virus IgG) ELISA Kit
ORB2307997
ReactivityHuman
Product group Assays
Overview
- SupplierBiorbyt
- Product NameHuman Anti-MPV IgG (Anti-Monkeypox Virus IgG) ELISA Kit
- Delivery Days Customer10
- Application Supplier Notestandard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-MPV IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-MPV IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-MPV IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human Anti-MPV IgG in the samples is then determined by comparing the OD of the samples to the standard curve
- Assay Detection Range3.13-200 ng/mL
- Assay Sensitivity1.39 ng/mL
- CertificationResearch Use Only
- Scientific DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-MPV IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-MPV IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-MPV IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human Anti-MPV IgG in the samples is then determined by comparing the OD of the samples to the standard curve.
- ReactivityHuman
- UNSPSC41116158