Human BCRP vesicle kit (HEK293 derived)
C4PVK006
Product group Assays
Overview
- SupplierCell4Pharma
- Product NameHuman BCRP vesicle kit (HEK293 derived)
- Delivery Days Customer9
- Applications SupplierVesicular transport assay
- Assay Test PrincipleKit can be used for substrate identification or inhibition screening. A master mix will be prepared, added to the membrane vesicles and incubated at 37℃ in the presence of ATP or AMP. Loaded vesicles will be filtered, lysed and eluted for detection.
- Assay Time2-3 hours
- Category SupplierAssay
- CertificationResearch Use Only
- Scientific DescriptionInside-out membrane vesicles are prepared from HEK293 cells with transiently overexpressed drug efflux transporters (ABC transporters) and used for high-throughput applications. The transporters translocate substrates into the membrane vesicles in an ATP-dependent manner. Detection of the substrate inside the vesicles is possible with radioactivity, fluorescence, or LC-MS detection, dependent on the substrate of choice.
- Shelf life instructionTwo years from the date of shipping
- Storage Instruction-80°C
- UNSPSC41116133
References
- Dankers, A. C., Sweep, F. C., Pertijs, J. C., Verweij, V., van den Heuvel, J. J., Koenderink, J. B., Russel, F. G., & Masereeuw, R. (2012). Localization of breast cancer resistance protein (Bcrp) in endocrine organs and inhibition of its transport activity by steroid hormones. Cell and tissue research, 349(2), 551–563. https://doi.org/10.1007/s00441-012-1417-5Read more
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