The chemotactic effect of GTX00416-pro Human C5a protein (active). Upper: (A)THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 without C5a was added in lower chamber, then cells in lower chamber were observed at low magnification (x100) after incubation for 3hrs. (B) THP-1 cells were seeded into the upper chambers and 50 ng/ml C5a was added in lower chamber, then cells in lower chamber were observed at low magnification (x100) after incubation for 3hrs. (C) THP-1 cells were seeded into the upper chambers and 100 ng/ml C5a was added in lower chamber, then cells in lower chamber were observed at low magnification (x100) after incubation for 3hrs. Lower: The number of migrated cells were counted at high magnification (x400) randomly (five fields for each filter).
The chemotactic effect of GTX00416-pro Human C5a protein (active). Upper: (A)THP-1 cells were seeded into the upper chambers and serum free RPMI-1640 without C5a was added in lower chamber, then cells in lower chamber were observed at low magnification (x100) after incubation for 3hrs. (B) THP-1 cells were seeded into the upper chambers and 50 ng/ml C5a was added in lower chamber, then cells in lower chamber were observed at low magnification (x100) after incubation for 3hrs. (C) THP-1 cells were seeded into the upper chambers and 100 ng/ml C5a was added in lower chamber, then cells in lower chamber were observed at low magnification (x100) after incubation for 3hrs. Lower: The number of migrated cells were counted at high magnification (x400) randomly (five fields for each filter).
Human C5a protein (active)
GTX00416-PRO
ApplicationsFunctional Assay
Product group Proteins / Signaling Molecules
Protein IDP01031
Overview
- SupplierGeneTex
- Product NameHuman C5a protein (active)
- Delivery Days Customer9
- ApplicationsFunctional Assay
- CertificationResearch Use Only
- ConjugateUnconjugated
- Protein IDP01031
- Protein NameComplement C5
- Scientific DescriptionThis gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the C5 alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mutations in this gene cause complement component 5 deficiency, a disease characterized by recurrent bacterial infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015]
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352202