GTX65635-pro CD40L protein (multimeric) does not need an enhancer to induce B cells activation. Method: PBL cells were incubated in 96-well plates (2x10 5 cells/well in 100μl RPMI supplemented with 10% FCS) for 24 hours at 37oC with the indicated concentration of CD40L (multimeric) or CD40L in the presence and absence of 1μg/ml Enhancer. Cells were washed with PBS and stained with CD86-PE antibody and CD19-FITC antibody, and then analyzed by flow cytometry.
GTX65635-pro CD40L protein (multimeric) does not need an enhancer to induce B cells activation. Method: PBL cells were incubated in 96-well plates (2x10 5 cells/well in 100μl RPMI supplemented with 10% FCS) for 24 hours at 37oC with the indicated concentration of CD40L (multimeric) or CD40L in the presence and absence of 1μg/ml Enhancer. Cells were washed with PBS and stained with CD86-PE antibody and CD19-FITC antibody, and then analyzed by flow cytometry.
Human CD40L / CD154 protein, DDDDK tag (active, multimeric)
GTX65635-PRO
ApplicationsFunctional Assay
Product group Proteins / Signaling Molecules
Overview
- SupplierGeneTex
- Product NameHuman CD40L / CD154 protein, DDDDK tag (active, multimeric)
- Delivery Days Customer9
- Application Supplier NoteInduces B cells activation (as demonstrated by dose-dependent upregulation of CD86) (ED50:: <1ng/ml).
- ApplicationsFunctional Assay
- CertificationResearch Use Only
- ConjugateUnconjugated
- Gene ID959
- Target nameCD40LG
- Target descriptionCD40 ligand
- Target synonymsCD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L, CD40 ligand, CD40 antigen ligand, CD40-L, T-B cell-activating molecule, T-cell antigen Gp39, TNF-related activation protein, tumor necrosis factor (ligand) superfamily member 5
- Storage Instruction-20°C
- UNSPSC41116120
- SpeciesHuman