Human CES1 protein, His tag

GeneTex

Human CES1 protein (active)

9

Carboxylesterase 1 (CES1) also known as Liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant human CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50 mM Tris (pH 7.5; the Assay Buffer), initiated by addition 50 microl of various concentrations of CES1 (dilute by assay buffer) to 50 microl of 2 mM Substrate 4-NPA (100mM stock in Acetone, dilute by deionized water). Incubated at 37C for 10min, then read at a wavelength of 400nm. One unit of enzyme activity is defined as the 1 microg of enzyme required to convert 1 pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37C. The specific activity of recombinant human CES1 is 1396 pmol/min/microg.

Research Use Only

Unconjugated

Lyophilized

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

-20°C or -80°C,2°C to 8°C

12352204