Human CXCL12 / SDF1 alpha ELISA Kit

Arigo Biolaboratories

Human CXCL12 / SDF1 alpha ELISA Kit

23

ELISA

CV:

62.5 pg/ml

No significant cross-reactivity or interference with Human beta-ECGF, GRO alpha, GRO beta, GRO gamma, MCP-1, MIP-1 alpha, MIP-1 beta, PTN, RANTES; mouse MIP-1 alpha, MIP-1 beta. The cross-reactivity tested with 50 ng/ml of Human SDF-1 beta is

4 hours

Research Use Only

Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. [provide by uniprot]

2°C to 8°C

41116133