WB analysis of GTX00224-pro Human FGF6 protein.
WB analysis of GTX00224-pro Human FGF6 protein.
Human FGF6 protein, His and GST tag
GTX00224-PRO
ApplicationsFunctional Assay
Product group Proteins / Signaling Molecules
Protein IDP10767
Overview
- SupplierGeneTex
- Product NameHuman FGF6 protein (active)
- Delivery Days Customer9
- Application Supplier NoteFibroblast growth factor 6 (FGF6) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. It may also play a importent role in muscle regeneration or differentiation. Besides, Fibroblast Growth Factor Receptor 1 (FGFR1) has been identified as an interactor of FGF6, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF6 and recombinant human FGFR1. Briefly, FGF6 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 microl were then transferred to FGFR1-coated microtiter wells and incubated for 2h at 37C. Wells were washed with PBST and incubated for 1h with anti-FGF6 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37C. Finally, add 50 microl stop solution to the wells and read at 450nm immediately. The binding activity of FGF6 and FGFR1 was in a dose dependent manner.
- ApplicationsFunctional Assay
- CertificationResearch Use Only
- ConjugateUnconjugated
- Protein IDP10767
- Protein NameFibroblast growth factor 6
- Scientific DescriptionThe protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene displayed oncogenic transforming activity when transfected into mammalian cells. The mouse homolog of this gene exhibits a restricted expression profile predominantly in the myogenic lineage, which suggested a role in muscle regeneration or differentiation. [provided by RefSeq, Jul 2008]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352202