Human GALP(Galanin-like peptide) ELISA Kit

Biorbyt

Human GALP(Galanin-like peptide) ELISA Kit

10

standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GALP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GALP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GALP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human GALP in the samples is then determined by comparing the OD of the samples to the standard curve

78.13-5000 pg/mL

28 pg/mL

Research Use Only

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galanin-like peptide(GALP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Galanin-like peptide(GALP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galanin-like peptide(GALP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Galanin-like peptide(GALP) in the samples is then determined by comparing the OD of the samples to the standard curve.

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