ELISA results of purified Goat anti-Human IgG F(c) antibody (min x Mouse serum proteins) tested against purified Human IgG F(c) (p/n 009-0103). Each well was coated in duplicate with 1.0 µg of Human IgG F(c) as well as Mouse IgG (p/n 010-0102). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Donkey anti-Goat IgG Antibody Peroxidase Conjugated (Min X Ch GP Ham Hs Ms Rb & Rt Serum Proteins) (p/n 605-703-125) and TMB substrate p/n TMBE-1000.