ELISA results of Purified Donkey Anti-Human IgG MinX10 Antibody Alkaline Phosphatase Conjugated tested against purified Human IgG mx10. Each well was coated in duplicate with 10µg of Human IgG (p/n 009-0102). The working dilution of Anti-Human IgG mx10 Alkaline Phosphatase Antibody is 1:34,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel Blocking buffer (p/n MB-066) and NPP Substrate (p/n NPP-100).