Binding of human IgG1kappa/APC to ICO-97-coated CML beads. Method: huIgG1kappa/APC was tested for binding to anti-human IgG (clone ICO-97)-coated 4micro beads in FACS (Inquire). Approximately 5 x 10^5 beads per tube were spun down and incubated 45 min at 4°C with 80microl of huIgG1kappa/APC at a 1:50 dilution (10microg/ml). Tubes were washed twice, fixed and analyzed by FACS. Beads stained positive with a mean shift of 3.45 log10 fluorescent units when compared to a buffer background. Binding was blocked when beads were pre-incubated 10 min with 20microl of 0.5 mg/ml unlabeled huIgG1kappa antibody (#ANC-295-010).