Sandwich ELISA analysis of human plasma using GTX60865 Human IgG4 antibody [RM120] as the capture antibody and GTX60920 Human Ig Light Chains antibody [RM129] (Biotin) as the detection antibody.
![Sandwich ELISA analysis of human IgG4 in monkey serum using GTX60865 Human IgG4 antibody [RM120] as the capture antibody and GTX60920 Human Ig Light Chains antibody [RM129] (Biotin) as the detection antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX60865/GTX60865_20200909_ELISA_141_w_23061123_551.webp "Sandwich ELISA analysis of human IgG4 in monkey serum using GTX60865 Human IgG4 antibody [RM120] as the capture antibody and GTX60920 Human Ig Light Chains antibody [RM129] (Biotin) as the detection antibody.")
![ELISA analysis of different amount of immunogen proteins using serial diluted GTX60865 Human IgG4 antibody [RM120].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60865/GTX60865_20200909_ELISA_36_w_23061123_190.webp "ELISA analysis of different amount of immunogen proteins using serial diluted GTX60865 Human IgG4 antibody [RM120].")
![IHC-P analysis of tonsil tissue using GTX60865 Human IgG4 antibody [RM120]. Dilution : 1:50,000](https://www.genetex.com/upload/website/prouct_img/normal/GTX60865/GTX60865_20200909_IHC-P_208_w_23061123_775.webp "IHC-P analysis of tonsil tissue using GTX60865 Human IgG4 antibody [RM120]. Dilution : 1:50,000")
![IHC-P analysis of human lymphoid tissue using GTX60865 Human IgG4 antibody [RM120].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60865/GTX60865_20200909_IHC-P_227_w_23061123_246.webp "IHC-P analysis of human lymphoid tissue using GTX60865 Human IgG4 antibody [RM120].")
![WB analysis of human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms using GTX60865 Human IgG4 antibody [RM120]. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.](https://www.genetex.com/upload/website/prouct_img/normal/GTX60865/GTX60865_20200909_WB_292_w_23061123_733.webp "WB analysis of human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms using GTX60865 Human IgG4 antibody [RM120]. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG.")
![ELISA analysis of monkey IgG using GTX60865 Human IgG4 antibody [RM120] and positive control Anti-monkey IgG antibody. The result shows that the GTX60865 does not react to monkey IgG.](https://www.genetex.com/upload/website/prouct_img/normal/GTX60865/GTX60865_210222_w_23061123_768.webp "ELISA analysis of monkey IgG using GTX60865 Human IgG4 antibody [RM120] and positive control Anti-monkey IgG antibody. The result shows that the GTX60865 does not react to monkey IgG.")
Sandwich ELISA analysis of human plasma using GTX60865 Human IgG4 antibody [RM120] as the capture antibody and GTX60920 Human Ig Light Chains antibody [RM129] (Biotin) as the detection antibody.
Rabbit Anti-Human IgG4 antibody [RM120]
GTX60865
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetIGHG4
Overview
- SupplierGeneTex
- Product NameHuman IgG4 antibody [RM120]
- Delivery Days Customer9
- Antibody SpecificityThis antibody reacts to the heavy chain of human IgG4, RM120 does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG3), and shows no cross reactivity to IgM, IgA, IgD, IgE. RM120 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat I
- Application Supplier NoteWB: 0.5 microg/mL - 2 microg/mL. ICC/IF: 1 microg/mL - 10microg/mL. IHC-P: 1 microg/mL - 10microg/mL. ELISA: 50ng/well - 200ng/well (for Capture) ; 0.05microg/mL - 0.2microg/mL (for Detection). *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRM120
- ConjugateUnconjugated
- Gene ID3503
- Target nameIGHG4
- Target descriptionimmunoglobulin heavy constant gamma 4 (G4m marker)
- Target synonymsimmunoglobulin heavy chain constant region gamma 4
- HostRabbit
- IsotypeIgG
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203