Human IL12A + IL27B fusion protein, His tag (active)

GeneTex

Human IL12A + IL27B fusion protein (active)

9

IL35 (Interleukin 35) is an IL-12 family cytokine, which is a dimeric protein composed of IL-12alpha and IL-27beta chains. IL35 is thought to mediate the immune inhibitory function of regulatory T cells and has been proven to promotes pancreas cancer growth through enhancement of proliferation and inhibition of apoptosis. Thus, proliferation assay of IL35 was conducted using PANC-1 cells. Briefly, PANC-1 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of recombinant human IL35. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 microl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37C. Proliferation of PANC-1 cells after incubation with IIL35 for 48h observed by inverted microscope. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with human recombinant IL35 for 48h, and IL35 significantly increased cell viability of PANC-1 cells.

Functional Assay

Research Use Only

Unconjugated

Interleukin-12 subunit alpha

-20°C or -80°C,2°C to 8°C

12352202