Human IL17E protein, His tag

GeneTex

Human IL17E protein (active)

9

IL25 (Interleukin-25), also known as IL17E, is reported to induce NF-kappaB activation, and stimulate the production of IL-8. Besides, this cytokine shares the sequence similarity with IL17, which is considered as a ligand for IL17RA. Thus, a binding ELISA assay was conducted to detect the association of recombinant human IL25 with IL17RA. Briefly, IL25 were diluted serially in PBS with 0.1% BSA (pH 7.4). Duplicate samples of 100 microl were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37C. Wells were washed with PBST and incubated for 1h with anti-IL25 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37C. Finally, add 50 microl stop solution to the wells and read at 450nm immediately. The binding activity of IL25 with IL17RA was in a dose dependent manner.

Functional Assay

Research Use Only

Unconjugated

Interleukin-25

The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

-20°C or -80°C,2°C to 8°C

12352202