Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) (3.47-0.05 nM). Coated protein was probed with IL18 antibody [HL1859] (GTX637584) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
![Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) (3.47-0.05 nM). Coated protein was probed with IL18 antibody [HL1761] (GTX637411) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX00227-pro/GTX00227-pro_822205898_20230324_ELISA_Indirect_2_23032819_767.webp "Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) (3.47-0.05 nM). Coated protein was probed with IL18 antibody [HL1761] (GTX637411) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.")
![Sandwich ELISA detection of recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) using antibodies as below. Capture: IL18 antibody [HL1859] (GTX637584) and IL18 antibody [HL1761] (GTX637411) (5 μg/mL) Detection: HRP-conjugated IL18 antibody [HL1859] (GTX637584), IL18 antibody [HL1761] (GTX637411) and IL18 antibody [HL2523] (GTX638884) (1 μg/mL) Please notice that needs to be conjugated to HRP to function as the detection antibody when paired with . Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX00227-pro/GTX00227-pro_822303551_20230915_ELISA_PAIR_Multiple_23091901_503.webp "Sandwich ELISA detection of recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) using antibodies as below. Capture: IL18 antibody [HL1859] (GTX637584) and IL18 antibody [HL1761] (GTX637411) (5 μg/mL) Detection: HRP-conjugated IL18 antibody [HL1859] (GTX637584), IL18 antibody [HL1761] (GTX637411) and IL18 antibody [HL2523] (GTX638884) (1 μg/mL) Please notice that needs to be conjugated to HRP to function as the detection antibody when paired with . Please contact us for custom HRP-conjugated antibody.")
![Sandwich ELISA detection of recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) using antibodies as below. Capture: IL18 antibody [HL2938] (GTX640322) (5 μg/mL) Detection: HRP-conjugated IL18 antibody [HL1859] (GTX637584) (1 μg/mL) Please notice that GTX637584 needs to be conjugated to HRP to function as the detection antibody when paired with GTX640322. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX00227-pro/GTX00227-pro_20240510_ELISA_PAIR_24051400_862.webp "Sandwich ELISA detection of recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) using antibodies as below. Capture: IL18 antibody [HL2938] (GTX640322) (5 μg/mL) Detection: HRP-conjugated IL18 antibody [HL1859] (GTX637584) (1 μg/mL) Please notice that GTX637584 needs to be conjugated to HRP to function as the detection antibody when paired with GTX640322. Please contact us for custom HRP-conjugated antibody.")
Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human IL18 protein, His tag (GTX00227-pro) (3.47-0.05 nM). Coated protein was probed with IL18 antibody [HL1859] (GTX637584) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
Human IL18 protein, His tag
GTX00227-PRO
ApplicationsFunctional Assay, ELISA
Product group Proteins / Signaling Molecules
Protein IDQ14116
Overview
- SupplierGeneTex
- Product NameHuman IL18 protein, His tag
- Delivery Days Customer9
- Application Supplier NoteRecommended antibody pairs for sandwich ELISA: Capture : GTX637584, Detection : GTX637411 or Capture : GTX637411, Detection : GTX637584. The detection antibody requires HRP conjugation. Please contact us for the conjugation service.
- ApplicationsFunctional Assay, ELISA
- CertificationResearch Use Only
- ConjugateUnconjugated
- Protein IDQ14116
- Protein NameInterleukin-18
- Scientific DescriptionThe protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352202
Datasheet
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