Indirect ELISA analysis was performed by coating the plate with recombinant Human IL6 protein, His tag (GTX135982-pro) (2634.91-41.32 pM). Coated protein was probed with IL6 antibody [HL2389] (GTX638598) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
![Sandwich ELISA detection of Human IL6 protein, His tag (GTX135982-pro) using ELISA pairs below. Pair 1: Capture: IL6 antibody [GT519-1] (GTX636573) (5 μg/mL) Detection: IL6 antibody [HL1168] (GTX636473) (1 μg/mL) Pair 2: Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL) Detection: IL6 antibody [HL1168] (GTX636473) (1 μg/mL)](https://www.genetex.com/upload/website/prouct_img/normal/GTX135982-pro/GTX135982-pro_44211_20230630_ELISA_PAIR_Multiple_23070401_398.webp "Sandwich ELISA detection of Human IL6 protein, His tag (GTX135982-pro) using ELISA pairs below. Pair 1: Capture: IL6 antibody [GT519-1] (GTX636573) (5 μg/mL) Detection: IL6 antibody [HL1168] (GTX636473) (1 μg/mL) Pair 2: Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL) Detection: IL6 antibody [HL1168] (GTX636473) (1 μg/mL)")
![Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below. Capture: IL6 antibody [GT519-1] (GTX636573) (5 μg/mL) Detection: IL6 antibody [HL2389] (GTX638598) (1 μg/mL)](https://www.genetex.com/upload/website/prouct_img/normal/GTX135982-pro/GTX135982-pro_44211_20230721_ELISA_PAIR_23072519_866.webp "Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below. Capture: IL6 antibody [GT519-1] (GTX636573) (5 μg/mL) Detection: IL6 antibody [HL2389] (GTX638598) (1 μg/mL)")
![Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below. Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL) Detection: IL6 antibody [HL2389] (GTX638598) (1 μg/mL)](https://www.genetex.com/upload/website/prouct_img/normal/GTX135982-pro/GTX135982-pro_44211_20230721_ELISA_PAIR_2_23072519_599.webp "Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below. Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL) Detection: IL6 antibody [HL2389] (GTX638598) (1 μg/mL)")
Indirect ELISA analysis was performed by coating the plate with recombinant Human IL6 protein, His tag (GTX135982-pro) (2634.91-41.32 pM). Coated protein was probed with IL6 antibody [HL2389] (GTX638598) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
Human IL6 protein, His tag
GTX135982-PRO
ApplicationsELISA
Product group Proteins / Signaling Molecules
Protein IDP05231
Overview
- SupplierGeneTex
- Product NameHuman IL6 protein, His tag
- Delivery Days Customer9
- Application Supplier NoteRecommended antibody pairs for sandwich ELISA: Capture : GTX636571 / GTX636573, Detection : GTX636473 / GTX638598.
- ApplicationsELISA
- CertificationResearch Use Only
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3569
- Target nameIL6
- Target descriptioninterleukin 6
- Target synonymsBSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6, interleukin-6, B-cell differentiation factor, B-cell stimulatory factor 2, CTL differentiation factor, hybridoma growth factor, interferon beta-2, interleukin BSF-2
- Protein IDP05231
- Protein NameInterleukin-6
- Scientific DescriptionThis gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
- Storage Instruction-20°C,-80°C
- UNSPSC41116120
- SpeciesHuman
Datasheet
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