Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (APOE4 HOM)

Axol Bioscience

Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (APOE4 HOM)

9

Neurons

Research Use Only

ax0111 Neural Stem Cells are derived from iPSCs generated from an 87-year-old female donor's dermal fibroblasts. They can be further differentiated to generate cortical excitatory neurons or striatal neurons.Key highlights include:• Derived from human iPSCs• Express the typical markers of cerebral cortical neural stem cells (PAX6, FOXG1 and nestin)• Spontaneously form polarized neural tube-like rosette structures when plated as a monolayer in culture• Can be differentiated into high-quality cortical excitatory neurons and striatal neuronsThe donor was diagnosed with Alzheimer's Disease at age 70 and has the APOE4 HOM (E4/E4) genotype. axoCells human iPSC-Derived Neural Stem Cells (NSCs) are derived from integration-free, episomally reprogrammed, induced pluripotent stem cells (iPSCs) under fully defined neural induction conditions. axoCells NSCs are easy to differentiate to neurons or mixed neural cell types, following our protocols and using our tailored media and reagent bundles. We provide Neural Maintenance Media (ax0031), BDNF (ax139800) and GDNF (ax139855) growth factors and NeurOne supplements (ax0674) to drive differentiation to cortical excitatory neurons, as well as Neural Plating Media (ax0033) and optimized SureBond-XF coating (ax0053), all as individual reagents or in kit format with the cells (ax5111). Our cortical excitatory neurons have been specifically developed for use in mono-, co- and tri-culture models of neurodegenerative disease and for drug discovery with microglia (ax0664), astrocytes (ax0704) and inhibitory interneurons (ax0662 or ax0667). The NSCs express typical markers of cerebral cortical neural stem and progenitor cells such as PAX6, FOXG1 and nestin, and spontaneously form polarized neural tube-like rosette structures when plated as a monolayer in culture. Additionally, axoCells NSCs are capable of generating a spectrum of cerebral cortical excitatory and striatal neurons that are electrically active and have the ability to form functional synapses and circuits in vitro. After thawing and plating, the neural stem cells terminally differentiate to acquire mature electrophysiological properties, and form functional synaptic networks over a period of ~ 20 days. Neurons derived from axoCells NSCs can be maintained in culture long-term (>1 year).

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