Human LTE4(Cysteinyl Leukotrienes E4) ELISA Kit

Biorbyt

Human LTE4(Cysteinyl Leukotrienes E4) ELISA Kit

10

standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human LTE4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LTE4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human LTE4 in the samples is then determined by comparing the OD of the samples to the standard curve

15.63-1000 pg/mL

5.26 pg/mL

Research Use Only

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cysteinyl Leukotrienes E4(LTE4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cysteinyl Leukotrienes E4(LTE4). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cysteinyl Leukotrienes E4(LTE4) in the samples is then determined by comparing the OD of the samples to the standard curve.

Human

41116158