Human MIP1 beta protein, His tag (active)

GeneTex

Human MIP1 beta protein (active)

9

Macrophage inflammatory protein-1beta (MIP-1beta), also known as CCL4 is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. MIP-1beta is a kind of chemotactic cytokine. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of MIP-1beta on the human T-lymphocyte leukemia cell line Jurkat. Briefly, Jurkat cells were seeded into the upper chambers (100 microl cell suspension, 5x105 cell/ml in RPMI-1640 with FBS free) and recombinant human MIP-1beta (0. 0001 ng/ml, 0. 001 ng/ml, 0. 1 ng/ml, 1 ng/ml, 10 ng/ml, 100 ng/ml diluted separately in serum free RPMI-1640) was added in lower chamber with a polycarbonate filter (8microm pore size) used to separate the two compartments. After incubation at 37C with 5% CO2 for 2h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (x100) and the number of migrated cells were counted at high magnification (x200) randomly. The recombinant human MIP-1beta is able to induce migration of Jurkat cells.

Functional Assay

Research Use Only

Unconjugated

C-C motif chemokine 4

The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. The encoded protein is secreted and has chemokinetic and inflammatory functions. [provided by RefSeq, Dec 2012]

-20°C or -80°C,2°C to 8°C

12352202