The chemotactic effect of GTX00424-pro Human MIP3 beta protein (active). Upper: (A) Jurkat cells were seeded into the upper chambers and serum free RPMI-1640 without MIP3b was added in lower chamber, then cells in lower chamber were observed at low magnification (x100) after incubation for 3hrs. (B) Jurkat cells were seeded into the upper chambers and serum free RPMI-1640 with 0.1 ng/ml MIP3b was added in lower chamber, then cells in lower chamber were observed at low magnification (x100) after incubation for 3hrs. Lower: The number of migrated cells were counted at high magnification (x400) randomly (five fields for each filter).
The chemotactic effect of GTX00424-pro Human MIP3 beta protein (active). Upper: (A) Jurkat cells were seeded into the upper chambers and serum free RPMI-1640 without MIP3b was added in lower chamber, then cells in lower chamber were observed at low magnification (x100) after incubation for 3hrs. (B) Jurkat cells were seeded into the upper chambers and serum free RPMI-1640 with 0.1 ng/ml MIP3b was added in lower chamber, then cells in lower chamber were observed at low magnification (x100) after incubation for 3hrs. Lower: The number of migrated cells were counted at high magnification (x400) randomly (five fields for each filter).
Human MIP3 beta protein, His tag (active)
GTX00424-PRO
ApplicationsFunctional Assay
Product group Proteins / Signaling Molecules
Protein IDQ99731
Overview
- SupplierGeneTex
- Product NameHuman MIP3 beta protein (active)
- Delivery Days Customer9
- Application Supplier NoteMacrophage Inflammatory Protein 3 Beta (MIP3b) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and Chemokine C-C motif ligand 19 (CCL19). This chemokine elicits its effects on its target cells by binding to the chemokine receptor chemokine receptor CCR7. It attracts certain cells of the immune system, including dendritic cells and antigen-engaged B cells, CCR7 central-memory T-Cells. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of recombinant human MIP3b on the Jurkat cell line. Briefly, Jurkat cells were seeded into the upper chambers (150 microl cell suspension,106 cells/ml in RPMI-1640 with FBS free) and MIP3b (0.01, 0.1, 1, 10, 100, and 1000 ng/ml diluted separately in serum free RPMI-1640) was added in lower chamber with a polycarbonate filter (8 microm pore size) used to separate the two compartments. After incubation at 37C with 5% CO2 for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (x100) and the number of migrated cells were counted at high magnification (x400) randomly (five fields for each filter). MIP3b is able to induce migration of Jurkat cells, and the migrated cells were counted at high magnification (x400). The optimum chemotaxis of recombinant human MIP3b occurs at 0.1-1 ng/ml.
- ApplicationsFunctional Assay
- CertificationResearch Use Only
- ConjugateUnconjugated
- Protein IDQ99731
- Protein NameC-C motif chemokine 19
- Scientific DescriptionThis antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7. [provided by RefSeq, Sep 2014]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352202