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ProductsBack/Human MMP-9 Antibody (OASB01327)

Human MMP-9 Antibody (OASB01327)

OASB01327
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMmp9
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    Human MMP-9 Antibody (OASB01327)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SB15c
  • Concentration
    0.5 mg/ml
  • Gene ID17395
  • Target name
    Mmp9
  • Target description
    matrix metallopeptidase 9
  • Target synonyms
    B/MMP9, Clg4b, Gel B, MMP-9, pro-MMP-9, matrix metalloproteinase-9, 92 kDa gelatinase, 92 kDa type IV collagenase, 92kD gelatinase, 92kD type IV collagenase, collagenase type IVB, gelatinase B
  • Host
    Mouse
  • Isotype
    IgG2a
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MMP-9 using anti-MMP-9 antibody (PB9669). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: NRK Whole Cell Lysate, Lane 2: ANA-1 Whole Cell Lysate, Lane 3: HEPA Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP-9 antigen affinity purified polyclonal antibody (Catalog # PB9669) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP-9 at approximately 78 kDa. The expected band size for MMP-9 is at 78 kDa.
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