Human MRPS12(28S ribosomal protein S12, mitochondrial) ELISA Kit
ORB2307976
ReactivityHuman
Product group Assays
Overview
- SupplierBiorbyt
- Product NameHuman MRPS12(28S ribosomal protein S12, mitochondrial) ELISA Kit
- Delivery Days Customer10
- Application Supplier Notestandard: 50 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MRPS12 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MRPS12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human MRPS12 in the samples is then determined by comparing the OD of the samples to the standard curve
- Assay Detection Range0.79-50 ng/mL
- Assay Sensitivity0.28 ng/mL
- CertificationResearch Use Only
- Scientific DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MRPS12 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MRPS12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human MRPS12 in the samples is then determined by comparing the OD of the samples to the standard curve.
- ReactivityHuman
- UNSPSC41116158