Human NF kappa B-p65 (Nuclear Factor Kappa B p65) ELISA Kit

Assay Genie

Human NF kappa B-p65 (Nuclear Factor Kappa B p65) ELISA Kit

12

ELISA

0.16-10 ng/mL

0.1 ng/mL

Research Use Only

Transcription factor p65

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NFkappaB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NFkappaB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NFkappaB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Human NFkappaB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.

2°C to 8°C

41116100

Human